| Titlu: | Assessment of the skin corrosion after application of a test element to reconstructed epidermis model – OECD 431 |
| Obiective: | To assess quantitatively the ability of a test element to produce a decrease in cell viability penetrating the stratum corneum by diffusion or erosion after different times of contact |
| Sistem de testare: | EPISKINTM with stratum corneum from human keratinocytes (3 batches – 1/week) |
| Planificare: | Duration of the study: 3 weeks Beginning: 4 weeks upon receipt of the sample Report: 2-3 weeks after the end of the study |
| Cantitatea de produs necesara: | 2 x 20 g |
| Metodologie: | Assessment of the cell viability by staining of the living cells with a vital dye (MTT). Measurementof the mithochondrial activity and reading of the optical densities |
| Procedura: | Per each reconstructed epidermis batch: D-1: • Control and incubation of the test systems upon receipt D1: • Preparation of the reconstructed epidermis (1/contact time) • Contact of the test element (3 minutes – 1 hour – 4 hours) • Rinsing of the test systems • Revelation of the cytotoxicity D2: • Reading |
Research and Experimentation Center
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