Titlu: | Assessment of the irritant potential of a test element on the EPISKIN(TM) reconstituted epidermis model – OECD 439 |
Obiective: | To assess quantitatively the ability of a test element to produce a decrease in cell viability penetrating the stratum corneum after contact for 15 minutes with the reconstituted epidermis |
Sistem de testare: | EPISKINTM with stratum corneum from human keratinocytes (3 units of epidermis) |
Planificare: | Duration of the study: 4 days Beginning: 4 weeks upon receipt of the sample Report: 2-3 weeks after the end of the study |
Cantitatea de produs necesara: | 2 x 20 g |
Metodologie: | Assessment of the percentage of cell viability by staining of the living cells with a vital dye (MTT). Measurement of the mithochondrial activity and reading of the optical densities |
Procedura: | Per each reconstructed epidermis batch: D-1: • Control and storage of the test systems upon receipt D1: • Preparation of the reconstructed epidermis • Contact of the test element (15 minutes) • Incubation for 42 hours D3: • Revelation of the cytotoxicity • Incubation for 3 hours • Reading |